Re: paraffin staining

From:Jamie Erickson

What species are you working in? You don't have enough information here? Human, Mouse,  monkey, type of fixation, length of fixation...what? It is very hard to stain formalin fixed CD4/CD8 in mouse tissues, I don't know about human. These markers are better stained in Frozen tissues. Please don't assume we all work with  Human tissue. 

Jamie Erickson

Scientist II

Wyeth/Genetics Institute

1 Burtt Rd.

Andover, MA   01810

work : (978) 247-1348

FAX  : (978) 247-1389

>>> Aneeta Patel  10/02 10:19 AM >>>

Hi all

Again i am hoping you guys can come thru for me.

I am looking to stain paraffin embedded tissues for cd4, cd8, cd3,cd28.

If anyone has antibody sources, procedures for this would be greatly appreciated.


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