Re: intestine cryosection problem
I have an acetone fixation method for frozen sections that has always worked
well for me. It results in excellent morphology, and no loss of sections.
70% Acetone - made with tap water!!
50% Acetone - made with tap water!!
Chill all solutions 4 degrees C.
1. Cut frozen sections, air dry for 10-15 minutes.
2. Fix in cold 100% Acetone for 10 minutes.
3. Air dry for 10-20 minutes.
4. Fix in cold 100% acetone for 10 minutes.
5. Place in cold 70% acetone for 30 seconds.
6. place in cold 50% acetone for 30 seconds.
7. Rinse in tap water.
8. Place in buffer.
You must use tap water - the ionic concentration is one of the key elements.
This procedure is from the old Biotek company. I mourn their demise.
I have great success with this fixation when using lymphoma markers,
cytokeratins, and most other antibodies. The only antibody that has not
worked with this fixation is TTF.
Hope this helps.
<< Previous Message | Next Message >>