Re: dpx mounting medium
Andrea Grantham, HT(ASCP asks about DPX.
It's a traditional synthetic, non-fluorescent, resinous
mounting medium, also sold as DePeX. The abbreviation
is from its composition:
D for Distrene-80, which was a make of polystyrene.
(presumably with M.W. 80,000).
P for plasticizer (either tri-o-cresyl phosphate
or dibutyl phthalate).
X for xylene, the solvent.
(Information from Lillie & Fullmer's "Histopathologic
Technic ... 1976)
DPX is very good, but sometimes you get a batch
with little droplets in it, and that's bad. I changed
over to a poly(methyl methacrylate) medium about 5
years ago (either Cytoseal or Entellan). DPX was rather
nicer to handle; when dried, it pealed off easily from
places it wasn't supposed to be.
-------------------------
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London, Canada N6A 5C1
kiernan@uwo.ca
http://publish.uwo.ca/~jkiernan/
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