Re: dpx mounting medium
Andrea Grantham, HT(ASCP asks about DPX.
It's a traditional synthetic, non-fluorescent, resinous
mounting medium, also sold as DePeX. The abbreviation
is from its composition:
D for Distrene-80, which was a make of polystyrene.
(presumably with M.W. 80,000).
P for plasticizer (either tri-o-cresyl phosphate
or dibutyl phthalate).
X for xylene, the solvent.
(Information from Lillie & Fullmer's "Histopathologic
Technic ... 1976)
DPX is very good, but sometimes you get a batch
with little droplets in it, and that's bad. I changed
over to a poly(methyl methacrylate) medium about 5
years ago (either Cytoseal or Entellan). DPX was rather
nicer to handle; when dried, it pealed off easily from
places it wasn't supposed to be.
John A. Kiernan
Department of Anatomy and Cell Biology
The University of Western Ontario
London, Canada N6A 5C1
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