Re: buffer solutions
On Fri, 5 Oct 2001, Susan Travers wrote:
> Does anyone know of a good reference for recipes and principles of
> making buffer solutions?
Here are two; both excellent. I think the the 2nd one is free.
The information is also in textbooks of general or physical
chemistry.
Perrin,DD & Dempsey,B 1974. Buffers for pH and metal ion
control. London: Chapman & Hall. p/b 176 pp.
ISBN 0 412 21890 9
Gueffroy,DE, ed. 1975. Buffers. A guide for the preparation and
use of buffers in biological systems. La Jolla,CA: Calbiochem.
p/b 24 pp.(Reprinted at least 10 times up to 1992 and probably
still being produced)
> ... It would also be of interest to know what
> the consequences of using different buffers (phosphate buffer, versus
> tris, versus acetate etc) are.
----------------------------------------
John A. Kiernan
Department of Anatomy & Cell Biology
The University of Western Ontario
London, Canada N6A 5C1
kiernan@uwo.ca
http://publish.uwo.ca/~jkiernan
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