Re: buffer solutions

From:"J. A. Kiernan"

On Fri, 5 Oct 2001, Susan Travers wrote:

> Does anyone know of a good reference for recipes and principles of 
> making buffer solutions? 

Here are two; both excellent. I think the the 2nd one is free.
The information is also in textbooks of general or physical

Perrin,DD & Dempsey,B 1974. Buffers for pH and metal ion
  control. London: Chapman & Hall. p/b 176 pp. 
  ISBN 0 412 21890 9
Gueffroy,DE, ed. 1975. Buffers. A guide for the preparation and
  use of buffers in biological systems. La Jolla,CA: Calbiochem.
  p/b 24 pp.(Reprinted at least 10 times up to 1992 and probably
  still being produced)

>                 ... It would also be of interest to know what 
> the consequences of using different buffers (phosphate buffer, versus 
> tris, versus acetate etc) are.
John A. Kiernan
Department of Anatomy & Cell Biology
The University of Western Ontario
London,  Canada   N6A 5C1

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