What precisely are you trying to demonstrate or measure?
As far as I am concerned a good H and E takes a lot of beating with
emphasis on using a dilute hematoxylin and a well differentiated eosin.
This will demonstrate reversal lines and osteoid very well
I think that especially for demonstrating osteoid, Sharpeys fibers that a
Heidenhain's azan takes a lot of beating. Having said that a Masson
trichrome generally can give you most details you want.
Of equal importance to the stain is the thickness of section. As thin a
section as possible is optimal for measuring osteoid thickness and
accurately delineating edges, while a thicker section (say 6-10 microns)
allows you to count osteoclasts, osteoblasts and bone lining cells more
At 04:00 PM 10/01/2001 -0500, you wrote:
>I have a really easy question and I feel silly asking it.... What is the
>best H&E and Trichrome stain for decalcified paraffin embedded tissue?
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