Re: Negative slide has specific staining
No mix up. They're frozen tissue. Slides are all of the same animal-smoke
exposed mice. The slides are all treated exactly the same until the
antibody is applied. All slides except the neg control get primary, the neg
gets IgG-My first thought was that I mixed up antibody and IgG, so I reran
it with the same results.
> -----Original Message-----
> From: Greg Dobbin [SMTP:dobbin@Upei.CA]
> Sent: Thursday, October 11, 2001 5:29 AM
> To: Berger, Jennifer
> Subject: Re: Negative slide has specific staining
> Hi Jennifer,
> What about a mix up prior to cutting? As in mislabelled fixation jars,
> or a mix up when trimming. Sounds like a mix up to me!?
> Date sent: Wed, 10 Oct 2001 18:55:00 -0600
> From: "Berger, Jennifer"
> Subject: Negative slide has specific staining
> Forwarded to: DOBBIN@acad1.cs.upei.ca
> To: "'email@example.com'"
> > Hi everyone
> > I have a strange problem here.
> > I'm doing some IHC for protein carbonyls on mouse lungs exposed to
> > smoke.
> > I'm blocking with goat serum.
> > My primary antibody is Rabbit Anti-DNP
> > My negative control is a slide that gets rabbit IgG instead of the
> > My secondary in biotinylated Goat Anti-Rabbit.
> > Okay, my problem is that the ONLY slide that shows any of what appears
> to be
> > specific staining (type 2 cells) is the negative slide (IgG instead of
> > antibody).
> > I've rerun it with the same results, so it's not that I got the slides
> > up.
> > Any ideas?
> > Thanks
> > Jennifer
> Greg Dobbin
> Pathology Lab
> Atlantic Veterinary College, U.P.E.I.
> 550 University Ave.
> Charlottetown, P.E.I.
> Canada, C1A 4P3
> Phone: (902)566-0744
> Fax: (902)566-0851
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