No mix up.  They're frozen tissue.  Slides are all of the same animal-smoke
exposed mice.  The slides are all treated exactly the same until the
antibody is applied.  All slides except the neg control get primary, the neg
gets IgG-My first thought was that I mixed up antibody and IgG, so I reran
it with the same results.
Jennifer
> -----Original Message-----
> From:	Greg Dobbin [SMTP:dobbin@Upei.CA]
> Sent:	Thursday, October 11, 2001 5:29 AM
> To:	Berger, Jennifer
> Subject:	Re: Negative slide has specific staining
> 
> Hi Jennifer,
> What about a mix up prior to cutting? As in mislabelled fixation jars, 
> or a mix up when trimming. Sounds like a mix up to me!?
> Greg
> 
> Date sent:      	Wed, 10 Oct 2001 18:55:00 -0600
> From:           	"Berger, Jennifer" 
> Subject:        	Negative slide has specific staining
> Forwarded to:   	DOBBIN@acad1.cs.upei.ca
> To:             	"'histonet@pathology.swmed.edu'"
> 
> 
> > 
> > Hi everyone
> > I have a strange problem here.
> > I'm doing some IHC for protein carbonyls on mouse lungs exposed to
> cigarette
> > smoke.
> > 
> > I'm blocking with goat serum.
> > My primary antibody is Rabbit Anti-DNP
> > My negative control is a slide that gets rabbit IgG instead of the
> primary.
> > My secondary in biotinylated Goat Anti-Rabbit.
> > 
> > Okay, my problem is that the ONLY slide that shows any of what appears
> to be
> > specific staining (type 2 cells) is the negative slide (IgG instead of
> > antibody).
> > I've rerun it with the same results, so it's not that I got the slides
> mixed
> > up.
> > Any ideas?
> > 
> > Thanks
> > Jennifer
> > 
> > 
> 
> 
> >
> >
> >
> >
> >
> >
> Greg Dobbin
> Pathology Lab
> Atlantic Veterinary College, U.P.E.I.
> 550 University Ave.
> Charlottetown, P.E.I.
> Canada,  C1A 4P3
> Phone: (902)566-0744
> Fax: (902)566-0851