Re: Negative slide has specific staining
What about a mix up prior to cutting? As in mislabelled fixation jars,
or a mix up when trimming. Sounds like a mix up to me!?
Date sent: Wed, 10 Oct 2001 18:55:00 -0600
From: "Berger, Jennifer"
Subject: Negative slide has specific staining
Forwarded to: DOBBIN@acad1.cs.upei.ca
> Hi everyone
> I have a strange problem here.
> I'm doing some IHC for protein carbonyls on mouse lungs exposed to cigarette
> I'm blocking with goat serum.
> My primary antibody is Rabbit Anti-DNP
> My negative control is a slide that gets rabbit IgG instead of the primary.
> My secondary in biotinylated Goat Anti-Rabbit.
> Okay, my problem is that the ONLY slide that shows any of what appears to be
> specific staining (type 2 cells) is the negative slide (IgG instead of
> I've rerun it with the same results, so it's not that I got the slides mixed
> Any ideas?
Atlantic Veterinary College, U.P.E.I.
550 University Ave.
Canada, C1A 4P3
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