Re: Negative slide has specific staining - me too!!
please could you also forward any replies on this subject to me too
as I have a very similar problem with non-immune rabbit IgG.
I do get a result with my primary antibody, but get the same with the
non-imm IgG. I reduced my primary anitbody conc, and the non-immune
conc to the same protein concentration as my primary. The staining
disappears on the primary ab slides than on the control ones.!
University of Birmingham
>I have a strange problem here.
>I'm doing some IHC for protein carbonyls on mouse lungs exposed to cigarette
>I'm blocking with goat serum.
>My primary antibody is Rabbit Anti-DNP
>My negative control is a slide that gets rabbit IgG instead of the primary.
>My secondary in biotinylated Goat Anti-Rabbit.
>Okay, my problem is that the ONLY slide that shows any of what appears to be
>specific staining (type 2 cells) is the negative slide (IgG instead of
>I've rerun it with the same results, so it's not that I got the slides mixed
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