Re: Kappa and Lambda
We had a major problem with background on Kappa/Lambda staining. We were
able to get rid of most of it by using ReVeal by Biocare Medical. We used
it in the pressure cooker as a heat retrieval method. It cleaned the slides
up nicely, including the control slides.
----- Original Message -----
From: "Smith, Sherry A. (Histology)"
Sent: Thursday, October 04, 2001 10:19 AM
Subject: Kappa and Lambda
> Help!! I am having a problem with background staining with Kappa and
> Lambda. I know what the problem is but do not know how to solve it. I am
> told that it is a IgG problem but how do you block out the background??
> current method is as follows: We use Cell Marque (monoclone) antibody.
> heat retrieval. We use Protease II for 8min. and incubate for 32min. It
> looks great except for the background staining. Does anyone have any
> Sherry Smith
> Presbyterian Hospital
> Charlotte, NC
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