Re: Kappa and Lambda

From:Jennifer MacDonald

We had a major problem with background on Kappa/Lambda staining.  We were
able to get rid of most of it by using ReVeal by Biocare Medical.  We used
it in the pressure cooker as a heat retrieval method.  It cleaned the slides
up nicely, including the control slides.

Jennifer MacDonald



----- Original Message -----
From: "Smith, Sherry A. (Histology)" 
To: 
Sent: Thursday, October 04, 2001 10:19 AM
Subject: Kappa and Lambda


> Help!!  I am having a problem with background staining with Kappa and
> Lambda.  I know what the problem is but do not know how to solve it.  I am
> told that it is a IgG problem but how do you block out the background??
Our
> current method is as follows:  We use Cell Marque (monoclone) antibody.
No
> heat retrieval.  We use Protease II for 8min. and incubate for 32min.  It
> looks great except for the background staining.  Does anyone have any
> suggestions???????
>
> Sherry Smith
> Presbyterian Hospital
> Charlotte, NC
>
>
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