Re: Kappa and Lambda
if you are staining human tissue, which i assume you are, on thing you can do is
put human serum in your protein block. i use 10%human serum in with the serum
from the host of the secondary and block with this for 20 before the primary
antibody and then again for 5 min. before the secondary, you can also put this
in with your secondary antibody.
"Smith, Sherry A. (Histology)" wrote:
> Help!! I am having a problem with background staining with Kappa and
> Lambda. I know what the problem is but do not know how to solve it. I am
> told that it is a IgG problem but how do you block out the background?? Our
> current method is as follows: We use Cell Marque (monoclone) antibody. No
> heat retrieval. We use Protease II for 8min. and incubate for 32min. It
> looks great except for the background staining. Does anyone have any
> Sherry Smith
> Presbyterian Hospital
> Charlotte, NC
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