From:Patti Loykasek

There are many different avenues you could pursue to start doing immunos.
Dako has a good reference manual that covers IHC staining in general. I am
sure that several different vendors would be happy to help you set up the
basics. You need to decide whether to go with predilutes or concentrated
antibodies based on your expected usage, and your technical knowledge. I
feel that in general, concentrates are best as you can titer for your
specifically processed tissue. Titers are really not that hard to make, and
a good sales rep should help you out with that. Check with your state
histology society for upcoming IHC workshops/seminars. Perhaps even get a
list of laboratories performing IHC and set up a mentoring relationship with
them. If I can answer specific procedural questions, please email me
directly. We perform many types of IHC in our lab, and I would be happy to
try and help. Good luck.

Patti Loykasek
Phenopath Laboratories
Seattle, WA

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