Re: IHC
On Mon, 1 Oct 2001, Ryan, Genoula K Ms USAMH wrote:
> To HistoNetters,
> Our new Chief of Pathology would like us to do a few immunos. We are not
> set up to do any. Is there a cheap way to do immunos? We don't want to
> purchase a Ventanna or anything fancy. Our workload is small and he only
> wants the basics (i.e., S100, CK, ER/PR). Any suggestions on how to get
> this up and running will be greatly appreciated. Also if there are any good
> reference books for IHC. Thanks in advance,
> Genie K. Ryan,
> Histology Supervisor
> HMEDDAC
> DSN: 371-2653
> 49-6221-172653
> Genoula.Ryan@hbg.amedd.army.mil
>
>
Genie,
For a cheap way to do immunos I highly recommend that you do them using
the Shandon Sequenza, (which is sort of by hand and not really.) You'll
need their racks and their coverplates. Then I highly recommend that you
use Dako concentrated antibodies for S-100, cytokeratins and ER/PR, their
detection systems and their pretreatment systems, particularly their "Target Retrieval System"
(TRS). Good Luck!
Dana Settembre
Immunohistochemistry Lab
Pathology Department
University Hospital
Newark, New Jersey
USA
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