From:Dana Settembre

On Mon, 1 Oct 2001, Ryan, Genoula K Ms USAMH wrote:

> To HistoNetters,
> Our new Chief of Pathology would like us to do a few immunos.  We are not
> set up to do any.  Is there a cheap way to do immunos?  We don't want to
> purchase a Ventanna or anything fancy.  Our workload is small and he only
> wants the basics (i.e., S100, CK, ER/PR).  Any suggestions on how to get
> this up and running will be greatly appreciated.  Also if there are any good
> reference books for IHC.  Thanks in advance,
> Genie K. Ryan,
> Histology Supervisor
> DSN: 371-2653
> 49-6221-172653
For a cheap way to do immunos I highly recommend that you do them using
the Shandon Sequenza, (which is sort of by hand and not really.) You'll
need their racks and their coverplates. Then I highly recommend that you
use Dako concentrated antibodies for S-100, cytokeratins and ER/PR, their
detection systems and their pretreatment systems, particularly their "Target Retrieval System"
(TRS).  Good Luck!

Dana Settembre
Immunohistochemistry Lab
Pathology Department
University Hospital
Newark,  New Jersey

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