Hi,
    Titer ... Titer ... Titer ... then when all else fails try a labeled
polymer like Envision (DAKO) or an endogenous biotin blocker. Most stromal
staining can be reduced through increasing the titer of the antibody.
Amos Brooks

----- Original Message -----
From: "Robert Embree" 
To: "'HistoNet Server'" 
Sent: Wednesday, October 10, 2001 3:05 PM
Subject: RE: IHC - Back ground stroma staining


> Hello all,
>
> Looking for suggestions on how to reduce back ground stroma staining of
> breast tissue with breast panel markers; er, pr, her2, ki-67, and p53.
>
> Thank you in advance,
>
> Robert
>
> Robert Embree
> Laboratory Operations
> Special Projects Coordinator
>
>        U S  L A B S
> The New Measure of Pathology
> ' (949) 788-9111
>            (800) 710-1800
> FAX    (800) 788-7751
> * Rembree@USLabs.net
> * www.uslabs.net
>
>