Re: Giemsa/HELP !!
Bruce Abaloz at the University of Melbourne, Australia asks:
>>after a stain for red/white blood cells on paraffin sections (Giemsa OR??)<<
Pathologists now pretty much rely on H & E in this situation. Effective
Giemsa staining of hematopoietic tissue really requires Zenker (chrome and
mercury) fixation which hardly anyone does any more. A special modification
of the Giemsa stain is also needed, sometimes with multiple dye baths and
differentiation (under microscopic control) with 10% colophonium rosin
(abietic acid) in alcohol. - I haven't seen this done (or done it myself)
since I was a resident in the late 60's.
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