Re: GMS stain on an H&E

From:Nancy E Weber

Hi Michele,

I've done this before at my previous post.  The residents would come up with
all kinds of weird requests.  Cut another slide, if you can, and start
fresh.  Do the GMS procedure first and then instead of the light green
counterstain, counterstain with the H&E.  From the last water step before
the light green go into Hematoxylin instead. Works pretty well.  Hope this

Nancy Weber
Research Associate
Veterinary Clinical Sciences
Ohio State University
----- Original Message -----
From: "Michelle D. Moore" 
To: "Histonet Server" 
Sent: Wednesday, October 10, 2001 10:03 AM
Subject: GMS stain on an H&E

> Hello fellow histonetters. Hope everyone is having a good morning. Quick
> question for you, my pathologist has requested me to do a GMS stain from
> of my H&E sections this is a first for me and I don't know how to go about
> this. The sample they collected for us was so small to begin with that
> when I did a GMS off the block it's not demonstrating what the doc is
> looking for. He says he can see some areas on the H&E that he can't
> distinguish between fungus and some other bacteria. HELP I have never
> this before and would like your input. Thank you in advance for you time
> help. I'm glad to have a place that I can go for help like this since I'm
> such a rural area. Have a wonderful day.

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