|From:||nina leek |
In theory, if you are presently incubating your primary Ab at 4 degrees overnight, 37 degrees for 4 hours should give you the same result. Binding reactions (as well as enyme reactions) tend to double in rate for every 10 degree rise in temperature.
That said, in practice, it dosen't always work quite like that. Some Abs (not many) are cold-requiring, and won't work at higher temperatures; a few will only work at higher temperatures. The doubling for every 10 degrees is only a rough guide, and only way to find out is to try it. Be sure you keep the slides moist.