RE: puckers/snout

From:"Georger, Mary"

You might want to try the technique that I use for eyes, where you have a
similar problem with the sclera expanding. Float your section out on a room
temperature water bath them dip them into a "hot" water bath, mine is about
45C, but that will be dependent on the melting point of your paraffin. If
the water bath is too hot the  section will wrinkle even worse than before
or "blow apart", so there is some fine tuning to the system. Also when you
dip into the hot water bath keep the angle of your slides at about 45
degrees to the surface of the water. I use the same technique for all of the
vessels that I am currently doing and it works like a charm.
Best of luck and if you have any questions feel free to contact me.
Mary Georger
University of Rochester Center for Cardiovascular Research

> ----------
> From: 	P. Emry
> Sent: 	Monday, October 8, 2001 6:58 PM
> To:
> Subject: 	puckers/snout
> Hi all,
> I am doing cross sections of snout to look at the nasal septum.  I run
> into the problem of having the septum swell when I soak it, but not the
> surrounding tissue.  There seems to be a capsul around the septum that
> does not expand on the waterbath so the septum puckers.  I have tried
> every paraffin trick I know.  Any advice?
> Trisha Emry
> U of Washington

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