Kreb's/Henseleit buffered salt solution is made up as follows from a
quite old recipe:-

	0.9%Sodium Chloride (0.154M)	.............. 94parts
	1.15% Potassium chloride (0.154M)    ...............   4parts
	1.22% Calcium chloride (0.11M)      ..................   3parts
	2.11% Potassium dihydrogen phosphate (0.154M) 1part
	3.82% Magnesium sulphate (0.154M)  ................. 1part
	1.42% Disodium hydrogen phosphate (0.1M) ......13parts
	1.3% Sodium bicarbonate (0.154M)	.................14parts
		
	Regards,
			Tony

	Tony Savage
	Histopathology Group
          Respiratory, Inflammation & Respiratory Pathogens CEDD	
         GlaxoSmithKline Medicines Research Centre,
	Gunnelswood Road,
	STEVENAGE,
	      Herts.
	   SG1  2NY

Tel.       +44  01438  764117
Fax.      +44  01438  764488
Email     TJS0817@gsk.com
mobile  +44 07753609835

> -----Original Message-----
> From:	Andrea Grantham [SMTP:algranth@u.arizona.edu]
> Sent:	Wednesday, October 10, 2001 5:03 PM
> To:	histonet@pathology.swmed.edu
> Subject:	need buffer "recipe"
> 
> Does anybody have a "recipe" to make up Krebb's buffer. I've already 
> consulted my collection of reference books and where I find many types of 
> buffers none go by this name.
> 
> Thanks!!
> 
> Andi
> .....................................................................
> : Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
> : Sr. Research Specialist       University of Arizona               :
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> : (FAX:  520-626-2097)          (email:  algranth@u.arizona.edu)       :
> :...................................................................:
>            http://www.cba.arizona.edu/histology-lab.html
>