Andrea
I found another one at
http://www.uclm.es/inabis2000/symposia/files/121/session.htm
The recipe is slightly different to the last one.
118mM NaCl, 4.7mM KCl, 1.0mM NaH2PO4, 1.2mM MgCl2, 1.3mM CaCl2, 25mM NaHCO3,
0.04mM EDTA and 11mM Glucose adjusted to pH 7.3-7.4 by aeration with 95%
oxygen and 5% carbon dioxide.
Regards
Roy Ellis
mailto:roy.ellis@imvs.sa.gov.au


> -----Original Message-----
> From: Andrea Grantham [mailto:algranth@u.arizona.edu]
> Sent: Thursday, 11 October 2001 1:33
> To: histonet@pathology.swmed.edu
> Subject: need buffer "recipe"
>
>
> Does anybody have a "recipe" to make up Krebb's buffer. I've already
> consulted my collection of reference books and where I find many types of
> buffers none go by this name.
>
> Thanks!!
>
> Andi
> .....................................................................
> : Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
> : Sr. Research Specialist       University of Arizona               :
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> : (voice:  520-626-4415)        Tucson, AZ  85724-5044    USA       :
> : (FAX:  520-626-2097)          (email:  algranth@u.arizona.edu)       :
> :...................................................................:
>            http://www.cba.arizona.edu/histology-lab.html
>
>