Phil,

in your experience with the antibody have you seen any correlation between
immunoreactive cells and macrophage cell morphology (ruffeled cytoplasmic
membrane borders, increase vacuolation, nuclear changes) after routine
staining techniques ?


regards,
rob


-----Original Message-----
From: Phil Bergin
To: Jenny Molde; histonet@pathology.swmed.edu
Sent: 10/1/01 3:42 AM
Subject: Re: Macrophages

We use the Dako clone Ber-MAC3 anti human CD163 which seems to be
working quite
well for identifying tissue resident macrophages.  Its supposed to be
good for
differentiating between non-activated and activated monocytes.

Philip Bergin
Göteborg Universitet
Göteborg, Sweden

Jenny Molde wrote:

> Dear Histonetters,
> We are currently working with polyurethane synthetic materials and are
> having problems with distinguishing between activated and non
activated
> macrophages. So far we have tried CD68, Ham 56.  Is there a specific
marker
> that would only pick up the above mentioned markers?  Any help would
be
> greatly appreciatted. Many thanks.
>
> Jenny Molde
> University of Cape Town
> South Africa




*************************************************************************** 
 The contents of this communication are intended only for the addressee and
may contain confidential and/or privileged material. If you are not the
intended recipient, please do not read, copy, use or disclose this
communication and notify the sender.  Opinions, conclusions and other
information in this communication that do not relate to the official
business of my company shall be understood as neither given nor endorsed by
it.  
***************************************************************************