There was an excellent workshop given at the NSH this year by Chris van der
Loos on double and triple staining methods. His slides were the best I have
ever seen. No background, crisp and clear color definition. He has a book
out called "Immunoenzyme Multiple Staining Methods" . It is available from
Springer-Velag, NY,www.springer-ny.com and BIOS Scientific Publishers,
Oxford,UK, www.bios.co.uk. If you are doing or going to begin double
staining, this is the book to have. Jenny

-----Original Message-----
From: rueggp [mailto:rueggp@earthlink.net]
Sent: Monday, October 08, 2001 11:12 AM
To: Engelhardt Peter
Cc: 'HistoNet Server'
Subject: Re: Double-labeling with Zymed Histostain DS Kit


Peter,
In the July issue of JOH Donald Van Hecke wrote a brilliant article on
double
labeling using DAKO Envision.
Patsy Ruegg

Engelhardt Peter wrote:

> Hello Histonets,
>
> has anybody experience in using the Zymed Histostain DS Kit or other
> Doublelabel-Kits?I want to detect two antigenes in the same cell (BrdU and
a
> viral protein)
> I tried doublestaining with 'Non-Kits' but my results were rather
> unassuming, but maybe it depends on the protocols I've used...
>
> Thanks a lot
>
> Peter
>
> Peter Engelhardt
> Institute of animal patholgy
> University of Bern
> Switzerland
> Email: peter.engelhardt@itpa.unibe.ch