Amoebae stain up really nicely with PAS stain but you can also
demonstrate them well with Heidenhains iron haematoxylin. I don't know of a
method that is specific for amoeba alone unless there is an antibody
available that would enable you to demonstrate them immunohistochemically. 
		Regards,
			Tony

	Tony Savage
	Histopathology Group
          Respiratory, Inflammation & Respiratory Pathogens CEDD	
         GlaxoSmithKline Medicines Research Centre,
	Gunnelswood Road,
	STEVENAGE,
	      Herts.
	   SG1  2NY

Tel.       +44  01438  764117
Fax.      +44  01438  764488
Email     TJS0817@gsk.com
mobile  +44 07753609835

> -----Original Message-----
> From:	Jasper, Thomas [SMTP:TJasper@smdc.org]
> Sent:	Thursday, October 04, 2001 3:58 PM
> To:	'histonet@pathology.swmed.edu'
> Subject:	Amoeba?
> 
> Hello everyone,
> 
> I had a pathologist ask me today if I knew of a procedure to specifically
> demonstrate amoeba. I've never heard of this, neither had she. I am
> speculating that they could show up with standard H+E, I really don't
> know.
> I would appreciate any feedback.
> 
> Thomas Jasper HT(ASCP) BAS
> Histology Team Leader
> SMDC Clinical Laboratory
> Duluth, MN
> tjasper@smdc.org
>