Amoebae stain up really nicely with PAS stain but you can also
demonstrate them well with Heidenhains iron haematoxylin. I don't know of a
method that is specific for amoeba alone unless there is an antibody
available that would enable you to demonstrate them immunohistochemically.
Respiratory, Inflammation & Respiratory Pathogens CEDD
GlaxoSmithKline Medicines Research Centre,
Tel. +44 01438 764117
Fax. +44 01438 764488
mobile +44 07753609835
> -----Original Message-----
> From: Jasper, Thomas [SMTP:TJasper@smdc.org]
> Sent: Thursday, October 04, 2001 3:58 PM
> To: 'firstname.lastname@example.org'
> Subject: Amoeba?
> Hello everyone,
> I had a pathologist ask me today if I knew of a procedure to specifically
> demonstrate amoeba. I've never heard of this, neither had she. I am
> speculating that they could show up with standard H+E, I really don't
> I would appreciate any feedback.
> Thomas Jasper HT(ASCP) BAS
> Histology Team Leader
> SMDC Clinical Laboratory
> Duluth, MN
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