Negative slide has specific staining

From:"Berger, Jennifer"

Hi everyone
I have a strange problem here.
I'm doing some IHC for protein carbonyls on mouse lungs exposed to cigarette

I'm blocking with goat serum.
My primary antibody is Rabbit Anti-DNP
My negative control is a slide that gets rabbit IgG instead of the primary.
My secondary in biotinylated Goat Anti-Rabbit.

Okay, my problem is that the ONLY slide that shows any of what appears to be
specific staining (type 2 cells) is the negative slide (IgG instead of
I've rerun it with the same results, so it's not that I got the slides mixed
Any ideas?


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