Fwd: Re: Giemsa/HELP !!

From:"Dr. Ian Montgomery"

        What's the World coming to, "hardly anyone uses Zenker." It's in my Fix cupboard next to the Susa and Corrosive formalin.

Date: Thu, 11 Oct 2001 08:00:13 -0400 (EDT)
From: RSRICHMOND@aol.com
Subject: Re: Giemsa/HELP !!
To: histonet@pathology.swmed.edu

Bruce Abaloz at the University of Melbourne, Australia asks:

>>after a stain for red/white blood cells on paraffin sections (Giemsa OR??)<<

Pathologists now pretty much rely on H & E in this situation. Effective
Giemsa staining of hematopoietic tissue really requires Zenker (chrome and
mercury) fixation which hardly anyone does any more. A special modification
of the Giemsa stain is also needed, sometimes with multiple dye baths and
differentiation (under microscopic control) with 10% colophonium rosin
(abietic acid) in alcohol. - I haven't seen this done (or done it myself)
since I was a resident in the late 60's.

Bob Richmond
Samurai Pathologist
Knoxville TN

Dr. Ian Montgomery,
Microscopy Service Unit,
Graham Kerr Building,
Institute of Biological & Life Sciences,
University of Glasgow,
G12 8QQ.
Tel: 0141 332 8855 Extn.6644.
e-mail: ian.montgomery@bio.gla.ac.uk

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