Fwd: Re: Giemsa/HELP !!
|From:||"Dr. Ian Montgomery" |
World coming to, "hardly anyone uses Zenker." It's in my Fix
cupboard next to the Susa and Corrosive formalin.
Date: Thu, 11 Oct 2001 08:00:13
Dr. Ian Montgomery,
Subject: Re: Giemsa/HELP !!
Bruce Abaloz at the University of Melbourne, Australia asks:
>>after a stain for red/white blood cells on paraffin sections
Pathologists now pretty much rely on H & E in this situation.
Giemsa staining of hematopoietic tissue really requires Zenker (chrome
mercury) fixation which hardly anyone does any more. A special
of the Giemsa stain is also needed, sometimes with multiple dye baths and
differentiation (under microscopic control) with 10% colophonium rosin
(abietic acid) in alcohol. - I haven't seen this done (or done it myself)
since I was a resident in the late 60's.
Microscopy Service Unit,
Graham Kerr Building,
Institute of Biological & Life Sciences,
University of Glasgow,
Tel: 0141 332 8855 Extn.6644.
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