Fast answer needed
I am doing some immunohistochemistry.
I get good results with the primary overnight but need to accelerate the
I need an incubation time of 4 hours for the primary antibody.
Which method would give me the staining in this time period?
Incubation @ 37°C or @ 4°C refrigerated?
I know this might not work. I need to know which will give me my best
Thanks in advance.
Carol Ann Bobrowitz
Physiology & BRI Histology Core Laboratory
Department of Physiology
Medical College of Wisconsin
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