Fast answer needed

From:Carol Ann Bobrowitz

I am doing some immunohistochemistry.
I get good results with the primary overnight but need to accelerate the

I need an incubation time of 4 hours for the primary antibody.
Which method would give me the staining in this time period?

Incubation @  37C  or  @ 4C refrigerated?

I know this might not work.  I need to know which will give me my best
Thanks in advance.
Carol Ann Bobrowitz
Physiology & BRI Histology Core Laboratory
Department of Physiology
Medical College of Wisconsin
Milwaukee, Wisconsin

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