FASTER ANSWER NEEDED
The antibodies are Receptors for TGF beta II 1:500 and TGF beta III
1:100 on rat tissue.
Detection HRP with DAB.
I have it working quite well with overnight room temperature
incubation. (very clean, no background)
My hunch was right, all the responses were leaning towards 37°C.
Now I'm thinking time should be cut.
Maybe 1 to 2 hour?
These glitches are not in my control! I'm getting ticked. I'm venting.
Since typing the above there have been changes, problems and
The primary will be Receptor TGF beta II 1:100.
I'm doing at 37°C.
It will be on 1 hour in the next five minutes.
Question 1 hour incubation or 1 1/2 hour incubation?
<< Previous Message | Next Message >>