Decalcified bone staining

From:Gayle Callis

Any H&E works well IF you control the decalcification so that nuclei are
not damaged by acids during decalcification.  By control, an endpoint test
(chemical test is easy to do) to determine when calcium is removed and
prevent overexposure of tissue to acid is how one would control

Eosin/Phloxine counterstain gives good tinctoral properties, but make sure
you do not overstain with this, cut back on time. Several companies now
sell this already made up, Richard Allan is one, Newcomer's is another

Classic Masson's Trichrome with aniline blue works very well. I used to let
my sections sit overnight in room temperature Bouins rather than use hot
Bouins, sections stayed on slide quite well.
Gayle Callis
Histopathology Supervisor
Veterinary Molecular Biology - Marsh Lab
Montana State University - Bozeman
19th and Lincoln St
Bozeman MT 59717-3610

406 994-6367
406 994-4303 (FAX)

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