I was wondereing if anyone has used the Novo castra CD34
antibody. I recently tryed the antibody at a 1:25 dilution
with a trypsin digestion and did achieve a strong staining
of vessels. When I tryed the same dilution of the antibody
1:25 and used a Citrate solution in a steamer for antigen
unmasking I achieved a good stain for the endothelial cells
but it appears as though some of my human fibroblast may
also be staining. Has anyone had a problem with CD34
staining their  human fibroblast?

kmajahad@tktx.com