tissue shrinkage

From:Nancy.Cardwell@mcmail.vanderbilt.edu

Dear Histonetters,

Once again we have an interesting question and I know someone out there has
a good answer.

Thanks in advance!!!

Any idea how much shrinkage from the original unfixed tissue size occurs
during paraffin embedding?

We are trying to do Morphometric measurements of a microvessel from inside
a human leg nerve.

We were under the impression that the dehydration step results in tissue
shrinkage of about 20%.  Does
anybody have a "reference" to shrinkage?

Nancy Cardwell
Vanderbilt University Medical Center
Department of Plastic Surgery Research
Nashville, TN  37232-6904     USA
615-322-7266 phone
615-343-2050 fax




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