help with quenching autofluorescence

From:xyz@wistar.upenn.edu (Zhao, Xinyu)

Dear histonetters,

I am working on a project using immunofluorescence staining to detect Ki-67 and HP1 antigen in mouse tissue.  I got positive result but the autofluorescence background (in both red and green) is severe.  And strangely, the staining patterns for those two antibodies are the same.  

 My questions:

1. How can I quench the autofluorescence (the tissue was formalin-fixed and paraffin-embedded)?

2. Could my staining with Ki67 and HP1 be false positive?


Any input will be great appreciated.

Jasmine X.Y. Zhao
The Wistar Insitute
Philadelphia






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