help with quenching autofluorescence
|From:||firstname.lastname@example.org (Zhao, Xinyu)|
I am working on a project using immunofluorescence staining to detect Ki-67 and HP1 antigen in mouse tissue. I got positive result but the autofluorescence background (in both red and green) is severe. And strangely, the staining patterns for those two antibodies are the same.
1. How can I quench the autofluorescence (the tissue was formalin-fixed and paraffin-embedded)?
2. Could my staining with Ki67 and HP1 be false positive?
Any input will be great appreciated.
Jasmine X.Y. Zhao
The Wistar Insitute
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