|From:||Frank Hogg <FHogg@dchsystem.com>|
Is anyone using antibodies for copper/zinc or manganese? For cu/zu I am using 1:100, for mn I am using 1:20 both are polyclonal ab's. I am also using heat retrieval. I am curious to know what others are doing. PBS vs Tris? I use sheep secondary, is there a better secondary for these? Any suggestions would be appreciated.