From:Frank Hogg <>

Is anyone using antibodies for copper/zinc or manganese? For cu/zu I am
using 1:100, for mn I am using 1:20 both are polyclonal ab's. I am also
using heat retrieval. I am curious to know what others are doing.  PBS vs
Tris? I use sheep secondary, is there a better secondary for these? Any
suggestions would be appreciated.

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