chromotrope 2R for eosinophil granules

From:Cindy Burchell <>

	Hello Histonetters,
    It's been a while since I have had the chance to be in contact with 
you, and now I need a favor again.  My bosses insist that I use something 
called Chromotrope 2R to counterstain eosinophilic granules after IHC 
staining MBP of eos.  I've found references, but never any specific 
methods.  I would be in debt to anyone who could spell out just how to 
handle this counter stain.   Thanks so much in advance for your time if you 
will help me in this matter!!!   Cindy B.

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