cell culture Sudan III vs Oil red O staining

From:Gayle Callis <uvsgc@msu.oscs.montana.edu>

I know a lady who did the Churukian Oil red O that was discussed last week
or so on cell cultures with excellent results (seen in Science magazine on
stem cell publications and advertisements in the past couple of years, her

It was less messy than a protocol with propylene glycol, easier to rinse
off excess stain from cell cultures, and stains beautifully with
hematoxylin counterstain with excellent brilliant red contrast to the blue

Oil Red O can also be purchased in small quantities from Aldrich, sister
company to Sigma, and maybe a bit cheaper from Fisher or VWR if you have a
term contract agreement from these companies.   

Gayle Callis
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717-3610
406 994-4705
406 994-4303

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