<P>Not sure this will help, but given that you have the same problem I have had in the past I can only tell you what I did and now haven't had any "visitors" to my storage containers.</P>
<P>1. I autoclave any of my carbouys after each use (DI water, some of my buffers and my retrieval one). Dry cycle 20 minutes. Bleach prior if it has been longer than a week since the last refill.</P>
<P>2. I switched to concentrated retrieval solutions commercially purchased. The change in cost was really very little when all was said and done actually. The most expensive one I have is the Target retrieval from Dako and since I don't use it routinely, it hasn't made a big impact in my budget. As to the sodium azide issue, my feeling is if you can avoid it, then do so.</P>
<P>3. I do refrigerate my retrieval solutions. I was lucky enough to find a spot in a huge walk in refrigerator and my boss did battle for me to keep it and got me a shelf as well. I don't really think that the refrigeration is all that critical if you use it as much as you do, but it won't hurt.</P>
<P>I honestly believe the autoclaving is what finally got the buggers to go bye-bye and the use of commercial products. In a pinch I've done the mix and still no critters though. </P>
<P>I don't think you are being lazy, just busy and no one wants to stop to make up stuff in the middle of a run or at a hairy point in the day. As a rule I set up the next days run the night before, but I've had days where that just isn't possible and it's a scramble the next morning. When you're a "one-man" show it isn't possible to be lazy and still get good results so don't be so hard on yourself.</P>
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