biotin blocks, in large volume

From:Gayle Callis <>

Have never seen it in large lots, but you could make it up yourself with
reagents from Sigma.  D-Biotin, 0.01% in buffer and avidin or strepavidin
(Elias book) 0.1% in buffer.

I am not sure I would like to reuse these reagents if made up in bulk.  

Just curious, what do you mean by large numbers of slides, a drop (approx
50 ul) per slide or section is usually sufficient, and what is sold by
Vector or DAKO usually does a lot of slides.

Gayle Callis
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717-3610
406 994-4705
406 994-4303

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