Re: catilage

>I am having a problem keeping my catilage sections attached to the slides
>during staining.  The slides being used are silane-prep slides from Sigma
>and the slides are put into a 40 C oven overnight.  What am I failing to do
>to maintain the material on the slides?


When I was doing a lot of cartilage sections I would first try white Elmers
glue in my floatation bath.  It really, really works.  Most of my cartilage
had some bone on it, which makes things a little easier.  For cryo sections
I used silanized slides activated with glutaraldehyde.  You always lose a
few no matter what you do due to the charge on the cartilage.

Randy Carpenter

Parker Hughes Institute
Roseville,  MN (home)

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