Re: Tinctorial stains for mitochondria
|From:||Rena Fail <RFail@charleston.net>|
You might want to try a Novelli's. It's quick and it's easy. N
hydrochloric acid (preheated to 60 degrees) for 3 minutes. Rinse in
distilled water. 1% acid fuchsin for seconds, rinse in distilled water, 1%
aqueous light green for app. 3 minuttes, rinse, dehydrate, clear, and mount.
Mitochondria stain purplish red and are surroundded by a green colored
peripheral wall. nuclear chromatin and collagen green, muscle tissue
purplish red,erythrocytes brilliant red.
REF. Thompson Selected Histochemical and Histopathology Method. 1966,
Charles C> Thomas, Publisher
Rena Fail AS,HT(ASCP)
Medical University of SC
At 04:20 PM 10/19/00 -0400, you wrote:
>I have some formalin fixed, formic acid decalcified tissues embedded in wax
>that I would like to section and stain for mitochondria. Does anyone know
>of any quick (non-immuno) staining methods that I could use. I have read
>that it is possible to detect them using an aniline acid-fuchsin technique,
>but I cannot find a supplier of this dye. Advice would be appreciated.
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