Re: Sialidase Digestion

Jeannette Mitchell at FAHC in Burlington, Vermont asks:

>>I have been asked to do an Alcian Blue with and without Sialidase 

To remove sialic acid (N-acetylneuraminic acid, NANA) from sections:

1. Deparaffinize sections and bring to water.

2. Sing NA NA Hey Hey Good-bye as loudly as you can for five minutes.

3. Proceed with Alcian blue stain.


     ;-)    ;-)    ;-)    ;-)    ;-)    ;-)    ;-)

SORRY ABOUT THAT FOLKS - I've been waiting to spring that one for about 25 

Bob Richmond
Samurai Pathologist
Knoxville TN

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