Re: RE: Oil Red O Question

From:David Pereira <>

The 72 hour Oil Red O will stain any residual lipids not removed by conventional processing.

>>> Sarah Christo <> 10/20 11:19 AM >>>
Dear Betsy,
   The only way I know to do ORO on paraffin sections is to do the osmic acid/potassium dichromate pre-treatment.  It is in the AFIP manual.

Sarah Christo, HT (ASCP)
Research Associate, Histology Lab
Texas A&M University
College of Veterinary Medicine
Dept. of Vet Anatomy & Public Health
College Station, TX  77843-4458
phone: (979) 845-3177
fax:  (979) 458-3499

>>> "Molinari, Betsy" <> 10/19/00 10:19AM >>>
Another ORO question. A pathologist wants ORO on paraffin sections. I
have found one protocol for this method in the AFIP manual. Has anyone
had any experience with this? It calls for the slides to be
deparaffinized and hydrated to water,then placed in absolute propylene
glycol for 3-5 mins. then stained in a 0.5%ORO solution for 48-72 hours.
Betsy Molinari
Texas Heart Institute

> ----------
> From: 	J. A. Kiernan[] 
> Sent: 	Thursday, October 19, 2000 10:13 AM
> To: 	Ryan.Linda
> Cc: 	'histonet'
> Subject: 	Re: Oil Red O Question
> On Wed, 18 Oct 2000, Ryan.Linda wrote:
> > I need to get frozen sections from liver sections which have been
> > stored in 10% NBF for some time now.  Will I run into any problems
> > freezing the livers at this stage?  Any tips on Oil Red O with the
> > pre-fixed frozen sections will be appreciated.
>   It would be sensible to soak the block in 30% sucrose overnight
>   in the fridge (until they sink) for cryoprotection. This will
>   minimize damage from ice crystals, which can be the cause
>   of numerous holes in frozen sections. Otherwise just follow
>   the standard procedure for staining with oil red O.
>  John A. Kiernan,
>  Department of Anatomy & Cell Biology,
>  The University of Western Ontario,
>  LONDON,  Canada  N6A 5C1

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