Re: Proliferation in rat

From:Robert Schoonhoven <>


We routinely do cell proliferation studies on rats and mice. I can't
answer your questions in detail at the moment as I am leaving on a
business trip later today.  If you can wait until Oct. 30th I can send
you the information that you would need.  

Answers to the following questions would help 
1- what organs are you interested in
2- are these archival tissues or have the animals yet to be treated
3- what were they fixed in and for how long (if known), or what are you
planning to fix them in
4- which part of the cell cycle are you wanting to look at

I am sure that the above information will allow me or someone else on
this list to help you.  

Kenneth Wester wrote:
> Hi All!
> Is there anyone who has experience from and/or suggestions on how to study
> proliferation in rat tissue, using immunohistochemistry. One problem is the
> crossreactivity between mouse and rat. Another is to find antibodies
> recognizing the "rat-epitope" for the respective proliferatio associated
> protein.
> Kenneth Wester
> Kenneth Wester, Ph.D.                   phone:  +46 (0)18 611 02 06
> Dept. of Genetics & Pathology           fax:    +46 (0)18 471 3432
> The Rudbeck laboratory
> Uppsala University
> Dag Hammarskjöldsväg 20
> S-752 37 Uppsala, Sweden

best regards,
Robert Schoonhoven
Laboratory of Molecular Carcinogenesis and Mutagenesis
Dept. of Environmental Sciences and Engineering
University of North Carolina
Chapel Hill, NC 27599
office 919-966-6343
   Lab 919-966-6140
   Fax 919-966-6123 

Don't go around saying the world owes you a living; the world owes you
nothing; it was here first. 
Mark Twain [Samuel Langhornne Clemens] (1835-1910)

<< Previous Message | Next Message >>