Re: Problem need help

From:Philip Oshel <>


Sorry I haven't replied sooner, but I'm going nuts trying to get a 
new computer configured and all of my files transferred. And set up 
new hardware. argh.

The information should be in the histnet archives. I'm sorry to say 
that, but I can't find my copy of the slush nitrogen post I sent.

Very briefly, in a calm time of the madness:
Fill a beaker with LN2
Place in a glass vacuum desiccator
Hook to a fast, high-volume rotary pump
Pump away until the LN2 starts forming slush
Vent to air, remove beaker carefully, and plunge freeze tissue, the 
smaller the pieces the better.

Let me know if you have any questions. I promise I'll do better!


>I know this was discussed a while ago but could someone please give me the
>instructions for the liquid nitrogen slush method of freezing tissue. As
>usual I need this info stat.
>Hey, I sure appreicate all you listeners out there hope you can help.
>Lynn Gardner

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