Re: Oil Red O Question

From:Jeffrey S Crews <>

Are you sure that the protocol is for *paraffin* sections? I don't
remember that from the AFIP manual, and the paraffin dehydration and
clearing process should wash out all the fat present.

Jeffrey Crews, HTL (ASCP)

On Thu, 19 Oct 2000 12:19:52 -0500 "Molinari, Betsy"
<> writes:
>Another ORO question. A pathologist wants ORO on paraffin sections. I
>have found one protocol for this method in the AFIP manual. Has 
>had any experience with this? It calls for the slides to be
>deparaffinized and hydrated to water,then placed in absolute 
>glycol for 3-5 mins. then stained in a 0.5%ORO solution for 48-72 
>Betsy Molinari
>Texas Heart Institute
>> ----------
>> From: 	J. A. Kiernan[]
>> Sent: 	Thursday, October 19, 2000 10:13 AM
>> To: 	Ryan.Linda
>> Cc: 	'histonet'
>> Subject: 	Re: Oil Red O Question
>> On Wed, 18 Oct 2000, Ryan.Linda wrote:
>> > I need to get frozen sections from liver sections which have been
>> > stored in 10% NBF for some time now.  Will I run into any 
>> > freezing the livers at this stage?  Any tips on Oil Red O with 
>> > pre-fixed frozen sections will be appreciated.
>>   It would be sensible to soak the block in 30% sucrose overnight
>>   in the fridge (until they sink) for cryoprotection. This will
>>   minimize damage from ice crystals, which can be the cause
>>   of numerous holes in frozen sections. Otherwise just follow
>>   the standard procedure for staining with oil red O.
>>  John A. Kiernan,
>>  Department of Anatomy & Cell Biology,
>>  The University of Western Ontario,
>>  LONDON,  Canada  N6A 5C1

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