Re: Oil Red O Question
|From:||Jeffrey S Crews <email@example.com>|
Are you sure that the protocol is for *paraffin* sections? I don't
remember that from the AFIP manual, and the paraffin dehydration and
clearing process should wash out all the fat present.
Jeffrey Crews, HTL (ASCP)
On Thu, 19 Oct 2000 12:19:52 -0500 "Molinari, Betsy"
>Another ORO question. A pathologist wants ORO on paraffin sections. I
>have found one protocol for this method in the AFIP manual. Has
>had any experience with this? It calls for the slides to be
>deparaffinized and hydrated to water,then placed in absolute
>glycol for 3-5 mins. then stained in a 0.5%ORO solution for 48-72
>Texas Heart Institute
>> From: J. A. Kiernan[SMTP:firstname.lastname@example.org]
>> Sent: Thursday, October 19, 2000 10:13 AM
>> To: Ryan.Linda
>> Cc: 'histonet'
>> Subject: Re: Oil Red O Question
>> On Wed, 18 Oct 2000, Ryan.Linda wrote:
>> > I need to get frozen sections from liver sections which have been
>> > stored in 10% NBF for some time now. Will I run into any
>> > freezing the livers at this stage? Any tips on Oil Red O with
>> > pre-fixed frozen sections will be appreciated.
>> It would be sensible to soak the block in 30% sucrose overnight
>> in the fridge (until they sink) for cryoprotection. This will
>> minimize damage from ice crystals, which can be the cause
>> of numerous holes in frozen sections. Otherwise just follow
>> the standard procedure for staining with oil red O.
>> John A. Kiernan,
>> Department of Anatomy & Cell Biology,
>> The University of Western Ontario,
>> LONDON, Canada N6A 5C1
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