On Wed, 18 Oct 2000, Ryan.Linda wrote:

> I need to get frozen sections from liver sections which have been
> stored in 10% NBF for some time now.  Will I run into any problems
> freezing the livers at this stage?  Any tips on Oil Red O with the
> pre-fixed frozen sections will be appreciated.

  It would be sensible to soak the block in 30% sucrose overnight
  in the fridge (until they sink) for cryoprotection. This will
  minimize damage from ice crystals, which can be the cause
  of numerous holes in frozen sections. Otherwise just follow
  the standard procedure for staining with oil red O.

 John A. Kiernan,
 Department of Anatomy & Cell Biology,
 The University of Western Ontario,
 LONDON,  Canada  N6A 5C1