Re: Oil Red O Question

From:Jeffrey S Crews <>

The NBF won't be a problem; it's actually supposed to help support the
fat in place. The added moisture might cause some ice crystal problems.
You can put the tissue in 2M sucrose overnight to help with that. 
As for the ORO itself, I like the propylene glycol technique. If you
filter before use (you have to use a vacuum filtration device like a
Buchner funnel), it's not any "dirtier" than the isopropanol technique,
plus you can be sure that you're not solubilizing any tiny fat deposits.
I've used it to stain lipid vacuoles inside cultured hepatocytes, and it
works very well.

Jeffrey Crews, HTL (ASCP)

On Wed, 18 Oct 2000 15:38:07 -0400 "Ryan.Linda" <>
>I need to get frozen sections from liver sections which have been
>stored in 10% NBF for some time now.  Will I run into any problems
>freezing the livers at this stage?  Any tips on Oil Red O with the
>pre-fixed frozen sections will be appreciated.
>Linda Ryan, BS, HT(ASCP)HTL
>National Institute of Environmental Health Sciences
>111  T. W. Alexander Dr.
>Bldg./rm.: 101/C-262
>P.O. Box 12233
>Research Triangle Park, NC 27709
>(919) 541-4880(Laboratory)

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