Re: Nuclear Fast Red

From:"Brothers, Rachel" <>

>Was wondering if Nuclear Fast Red can be cleared in Xylene for
>coverslipping.  I've never worked with the stuff, and had a co-worker asking
>me some IHC questions.  Want to make sure I gave good advice.

The answer is, yes, NFR can be cleared in xylene. It is one of my favorite
counterstains. It's quick and I prefer the contrast to hematoxylin when I use a
DAB-black chromogen. I use Nuclear Fast Red from Vector, but just tell your
co-worker to filter it first. 

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