Re: Nuclear Fast Red

From:"J. A. Kiernan" <>

On Mon, 23 Oct 2000, Bennett, Catherine (Katie) wrote:

> Was wondering if Nuclear Fast Red can be cleared in Xylene for
> coverslipping.

  Yes. It resists alcohol and xylene, but if this is to
  counterstain something immunohistochemical, make sure
  your principal end-product is also insoluble. The DAB
  oxidation product is, but most alkaline phosphatase
  end products are extracted by alcohol or xylene.

 John A. Kiernan,
 Department of Anatomy & Cell Biology,
 The University of Western Ontario,
 LONDON,  Canada  N6A 5C1

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