Re: Immuno Staining and Xylene Substitute
From: | Julie Collins <jcollins@kumc.edu> |
I used to use Hemo-De (citrus-based) to coverslip with. Once, in a hurry to dry the slides to send them out, I put them in a 70 degree oven on slide trays (which is the way the routine Histo Lab dries their slides before filing them). When I took them out of the oven, the Hematoxylin and DAB were both gone. I don't know the reason why this happened, but I wasn't anxious to see if it would happen again.
Julie
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