Re: IHC background question
|From:||Jan Shivers <email@example.com>|
I work in a veterinary diagnostic laboratory and run across background
staining in various species from time to time. When that happens I add
normal serum from the particular "case" species to the biotinylated
secondary reagent (2% normal serum per volume secondary Ab). It usually
does the trick.
Working with hyperimmune serum as your antibody source is tricky, though.
Oftentimes you have to use it at such a high concentration, since the
antibodies are in short supply, so there is bound to be background caused by
the other serum antibodies present in the mix.
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