Re: Fwd: Re: Tinctorial stains for mitochondria
|From:||Rena Fail <RFail@charleston.net>|
At 10:22 AM 10/20/00 +0100, you wrote:
> Interesting method. Any mention of the preferred fixative.
> Date: Thu, 19 Oct 2000 20:59:09 -0400
> Subject: Re: Tinctorial stains for mitochondria
> Cc: email@example.com
> hydrochloric acid (preheated to 60 degrees) for 3 minutes. Rinse in
> distilled water. 1% acid fuchsin for seconds, rinse in distilled water, 1%
> aqueous light green for app. 3 minuttes, rinse, dehydrate, clear, and mount.
> Mitochondria stain purplish red and are surroundded by a green colored
> peripheral wall. nuclear chromatin and collagen green, muscle tissue
> purplish red,erythrocytes brilliant red.
> REF. Thompson Selected Histochemical and Histopathology Method. 1966,
>> Thomas, Publisher
> Rena Fail AS,HT(ASCP)
> Medical University of SC
> At 04:20 PM 10/19/00 -0400, you wrote:
>>I have some formalin fixed, formic acid decalcified tissues embedded in wax
>> Does anyone know
>> I have read
>>that it is possible to detect them using an aniline acid-fuchsin technique,
>> Advice would be appreciated.
> Dr. Ian Montgomery,
> West Medical Building,
> University of Glasgow,
> G12 8QQ.
> Fax: 0141 330 2923
> e-mail: firstname.lastname@example.org
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