10%NBF

At 10:22 AM 10/20/00 +0100, you wrote:
> Rena,
>         Interesting method. Any mention of the preferred fixative.
> Ian.
> 
> 
> Date: Thu, 19 Oct 2000 20:59:09 -0400
><>
> Subject: Re: Tinctorial stains for mitochondria
><>
> Cc: histonet@pathology.swmed.edu
> 
>  Darren,
> 
>       N
> hydrochloric acid (preheated to 60 degrees) for 3 minutes. Rinse in
> distilled water. 1% acid fuchsin for seconds, rinse in distilled water, 1%
> aqueous light green for app. 3 minuttes, rinse, dehydrate, clear, and mount.
> Mitochondria stain purplish red and are surroundded by a green colored
> peripheral wall. nuclear chromatin and collagen green, muscle  tissue
> purplish red,erythrocytes brilliant red.
> REF. Thompson Selected Histochemical and Histopathology Method. 1966,
>> Thomas, Publisher
> 
> 
> Rena Fail AS,HT(ASCP)
> Medical University of SC
> Charleston,Sc
> 
> 
> 
> 
> 
> 
> At 04:20 PM 10/19/00 -0400, you wrote:
>>I have some formalin fixed, formic acid decalcified tissues embedded in wax
>>  Does anyone know
>>  I have read
>>that it is possible to detect them using an aniline acid-fuchsin technique,
>>  Advice would be appreciated.
>>
>>Darren.
>>
>>
>>
>>
>  Dr. Ian Montgomery,
> West Medical Building,
> University of Glasgow,
> Glasgow,
> G12 8QQ.
>  Extn:6602.
> Fax: 0141 330 2923
> e-mail: ian.montgomery@bio.gla.ac.uk