Re: Cedar wood oil and Carnoy's
From: | "L. Gibbs" <lgibbs@u.washington.edu> |
Hi Rebecca-
You can use Xylene to clear Carnoy's fixed tissue. You could try
three changes of 100% ETOH (1 hour each) followed by 2 hours in
Xylene. You may be able to use the limonene-based clearants in
order to avoid brittle specimens.
I don't often use Carnoy's but I do often process for
Methacarn's.
Just in case you could use a schedule for Methacarn's:
mMethacarn's fixation 3-24 hours, MeOH x 3(1 hour each), Methyl
Benzoate 1 hour, Xylene x 2 (45 minutes each), Paraffin x 2 (1
hour each).
Good luck!
Lorraine Gibbs
Physiology & Biophysics
University of Washington
On Fri, 20 Oct 2000, Rebecca S Smith wrote:
> We are doing a research project for mast cells. Of course all the readings
> that the researching scientist has read say that Carnoy's fixation is the
> "BEST" way for preserving the mast cell for later
> demonstration. Soooooooo, I've conceded to this idea. OK, we'll use
> Carnoy's. Now we get to the readings on processing tissues following
> Carnoy's fixation and we get all these spotty directions on the use of
> Cedar Wood Oil. (Slimy, Sticky, Gooey STUFF). OK, we'll use Cedar Wood
> Oil. What a mess! As before mentioned, spotty directions. Some
> references have mentioned using "thin Cedar Wood Oil". No mention of how
> thin, no mention of what to thin it with. The processing following Cedar
> Wood Oil is not really clear.
> ANYWAY! My real question for you Histo Gurus out there is
> this: Why? What is the purpose? Did someone get this crazy idea because
> they had some Cedar Wood Oil in the back closet (for use on the family
> heirloom) and it worked and now it's gospel on how to do this Carnoy's
> processing? I've read some references that just mention using "thin cedar
> wood oil" for storage of wet tissues and not using it if you're processing
> right away!
> If someone can save me from using this stuff, by giving me real referenced
> reasoning for not using it, I will forever be in your debt! Do you like
> Cookies? Chocolate Chip? Peanut Butter? HELP!!!
>
>
>
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